KMID : 0903519890320040374
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology 1989 Volume.32 No. 4 p.374 ~ p.377
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Attrition Effect of Beads on Enzymatic Saccharification of Raw Starch
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Abstract
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To optimize the enzymatic saccharification of raw-starch, reaction conditions by shaking with glass beads were adapted together with ¥á-amylase from Streptorrayces sp. 4M-2 and amyloglucosidase from commercial source. When raw-starch was degraded by the ¥á-amylase in shaking flask with beads (raw-starch : bead in diam. of 3§®=1 : 5 by weight) at the shaker speed of 300rpm, the saccharification rate of corn and potato starch were increased up to 88% and 69% after 30 hrs of reaction, respectively. Application of the amyloglucosidase in combination with the ¥á-amylase enhanced the rate of saccharifcation: 88% of saccharification was obtained in 6 hrs for raw-corn starch under the same reaction conditions as above.
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